Figure 1 shows that AMD alignment preserves mitochondrial membranes even across a severe curtaining artifact. In a user study (n=4 expert annotators), NemoCeph 13 reduced total correction time by 82% compared to manual TrakEM2. NemoCeph 13 advances the state of the art by tightly coupling registration and segmentation with human-in-the-loop learning. The adaptive mesh effectively handles FIB-specific artifacts that previous methods could not. A limitation is the requirement for ≥5% labeled data when fine-tuning; future work will explore zero-shot segmentation using foundation models (e.g., SAM-EM).
[Your Name/Team], [Affiliation], [ORCID] software nemoceph 13
| Tool | Alignment error (px) | Segmentation ARI | Proofreading time (hr/100µm³) | |-------------------|----------------------|------------------|-------------------------------| | EM-Align | 1.23 ± 0.45 | 0.76 | 12.4 | | BigWarp (manual) | 0.89 ± 0.33 | N/A (manual) | 28.1 | | | 0.48 ± 0.12 | 0.93 | 2.6 | Figure 1 shows that AMD alignment preserves mitochondrial