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Primer Premier

Setool Setup May 2026

Primer Premier's search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in a ranked order. Load the gene of interest from NCBI, select a search range, sit back and let Primer Premier pick the best possible primers for you.

setool setup



Setool Setup May 2026

@click.group() def main(): """SEtool - Security Enhancement Tool""" pass

# setup.py import os import sys import json import shutil import subprocess from pathlib import Path from typing import Dict, List, Optional

def main(): """Entry point for setup script""" import argparse parser = argparse.ArgumentParser(description="SETool Setup Utility") parser.add_argument("--config", help="Path to configuration file") parser.add_argument("--skip-deps", action="store_true", help="Skip dependency installation") parser.add_argument("--force", action="store_true", help="Force reinstall") parser.add_argument("--verify-only", action="store_true", help="Only verify installation") args = parser.parse_args() setup = SEToolSetup(args.config) if args.verify_only: setup.verify_installation() else: success = setup.run(skip_deps=args.skip_deps, force=args.force) sys.exit(0 if success else 1) setool setup

# Import and run main CLI try: from setool.cli import main sys.exit(main()) except ImportError as e: print(f"Error importing SETool: {{e}}") print("Please run 'python setup.py' to complete installation") sys.exit(1) ''' script_path.write_text(script_content) script_path.chmod(0o755) print(f"✓ Executable created at {script_path}") # Add to PATH (Linux/macOS) if sys.platform != "win32": bashrc = Path.home() / ".bashrc" if bashrc.exists(): with open(bashrc, 'a') as f: f.write(f'\nexport PATH="$PATH:{self.home_dir}"\n') print("✓ Added to PATH in .bashrc") return True except Exception as e: print(f"✗ Failed to create executable: {e}") return False def verify_installation(self) -> Dict: """Verify installation completeness""" print("\n🔍 Verifying installation...") checks = { "directories": all(Path(d).exists() for d in [ self.setup_config["installation_dir"], self.setup_config["data_dir"], self.setup_config["logs_dir"] ]), "config_file": self.config_file.exists(), "env_file": (self.home_dir / ".env").exists(), "executable": (self.home_dir / "setool").exists() } for check, passed in checks.items(): status = "✓" if passed else "✗" print(f"{status} {check}: {'OK' if passed else 'FAILED'}") return checks def save_config(self) -> bool: """Save configuration to file""" try: self.home_dir.mkdir(exist_ok=True) with open(self.config_file, 'w') as f: json.dump(self.setup_config, f, indent=4) print(f"✓ Configuration saved to {self.config_file}") return True except Exception as e: print(f"✗ Failed to save configuration: {e}") return False def run(self, skip_deps: bool = False, force: bool = False) -> bool: """Main setup routine""" print("🚀 SETool Setup - Professional Installation\n") print("=" * 50) if force and self.config_file.exists(): print("⚠️ Force mode: Reinstalling...") shutil.rmtree(self.home_dir, ignore_errors=True) # Step 1: Create directories print("\n📁 Creating directories...") if not self.create_directories(): return False # Step 2: Install dependencies if not skip_deps: if not self.install_dependencies(): print("⚠️ Dependency installation failed, continuing...") # Step 3: Setup environment print("\n🌍 Setting up environment...") if not self.setup_environment_variables(): return False # Step 4: Create executable print("\n🔧 Creating executable...") if not self.create_executable_script(): return False # Step 5: Save configuration self.setup_config["setup_complete"] = True if not self.save_config(): return False # Step 6: Verify verification = self.verify_installation() if all(verification.values()): print("\n✅ SETool setup completed successfully!") print(f"\n📍 Installation location: {self.home_dir}") print(f"📝 Configuration file: {self.config_file}") print("\n💡 Next steps:") print(" 1. Restart your terminal or run: source ~/.bashrc") print(" 2. Run 'setool --help' to get started") return True else: print("\n❌ Setup completed with warnings. Please check the verification output.") return False

if == " main ": main()

# Load environment env_file = setool_home / ".env" if env_file.exists(): with open(env_file) as f: for line in f: if "=" in line: key, value = line.strip().split("=", 1) os.environ[key] = value

# Add SETool to path setool_home = Path("{self.home_dir}") sys.path.insert(0, str(setool_home.parent)) @click

@main.command() def status(): """Show setup status""" console.print("[green]✓ SETool is properly configured[/green]")


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